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rt pcr buffer  (TaKaRa)


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    Structured Review

    TaKaRa rt pcr buffer
    Rt Pcr Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 25222 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rt+pcr+buffer/Premix+Ex+Taq/10__3390_slash_jof12040282-85-12-22
    Average 99 stars, based on 25222 article reviews
    rt pcr buffer - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: FcLRR1 Regulates Hyphal Growth and Plant Infection in Fusarium circinatum
    Article Snippet: The concentration of cDNA was adjusted to 500 μg/μL, as determined by DNA fluorometry. qPCR was performed via TB Green Premix Ex Taq II (Tli RNaseH Plus) (TaKaRa, Dalian, China). .. The qPCR system consisted of the following components: 12.5 μL of 2× RT–PCR buffer (containing SYBR Green and Premix Ex Taq from TaKaRa), 0.5 μL of both the forward and reverse primers (each at 20 μM), 2 μL of genomic DNA, and 9.5 μL of ddH2O. .. The quantitative real-time PCR was carried out on a Mastercycler (Eppendorf, Carlsbad, CA, USA) with the following thermal cycling conditions: initial denaturation at 95 ◦C for 2 min, followed by 40 cycles of 95 ◦C for 10 s and 60 ◦C for 20 s, ending with a melting curve step.

    Article Title: FcLRR1 Regulates Hyphal Growth and Plant Infection in Fusarium circinatum
    Article Snippet: The concentration of cDNA was adjusted to 500 μg/μL, as determined by DNA fluorometry. qPCR was performed via TB Green Premix Ex Taq II (Tli RNaseH Plus) (TaKaRa, Dalian, China). .. The qPCR system consisted of the following components: 12.5 μL of 2× RT–PCR buffer (containing SYBR Green and Premix Ex Taq from TaKaRa), 0.5 μL of both the forward and reverse primers (each at 20 μM), 2 μL of genomic DNA, and 9.5 μL of ddH 2 O. .. The quantitative real-time PCR was carried out on a Mastercycler (Eppendorf, Carlsbad, CA, USA) with the following thermal cycling conditions: initial denaturation at 95 °C for 2 min, followed by 40 cycles of 95 °C for 10 s and 60 °C for 20 s, ending with a melting curve step.

    SYBR Green Assay:

    Article Title: FcLRR1 Regulates Hyphal Growth and Plant Infection in Fusarium circinatum
    Article Snippet: The concentration of cDNA was adjusted to 500 μg/μL, as determined by DNA fluorometry. qPCR was performed via TB Green Premix Ex Taq II (Tli RNaseH Plus) (TaKaRa, Dalian, China). .. The qPCR system consisted of the following components: 12.5 μL of 2× RT–PCR buffer (containing SYBR Green and Premix Ex Taq from TaKaRa), 0.5 μL of both the forward and reverse primers (each at 20 μM), 2 μL of genomic DNA, and 9.5 μL of ddH2O. .. The quantitative real-time PCR was carried out on a Mastercycler (Eppendorf, Carlsbad, CA, USA) with the following thermal cycling conditions: initial denaturation at 95 ◦C for 2 min, followed by 40 cycles of 95 ◦C for 10 s and 60 ◦C for 20 s, ending with a melting curve step.

    Article Title: FcLRR1 Regulates Hyphal Growth and Plant Infection in Fusarium circinatum
    Article Snippet: The concentration of cDNA was adjusted to 500 μg/μL, as determined by DNA fluorometry. qPCR was performed via TB Green Premix Ex Taq II (Tli RNaseH Plus) (TaKaRa, Dalian, China). .. The qPCR system consisted of the following components: 12.5 μL of 2× RT–PCR buffer (containing SYBR Green and Premix Ex Taq from TaKaRa), 0.5 μL of both the forward and reverse primers (each at 20 μM), 2 μL of genomic DNA, and 9.5 μL of ddH 2 O. .. The quantitative real-time PCR was carried out on a Mastercycler (Eppendorf, Carlsbad, CA, USA) with the following thermal cycling conditions: initial denaturation at 95 °C for 2 min, followed by 40 cycles of 95 °C for 10 s and 60 °C for 20 s, ending with a melting curve step.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Digestion of chrysanthemum stunt viroid by leaf extracts of Capsicum chinense indicates strong RNA-digesting activity.
    Article Snippet: Key message CSVd could not infect Nicotiana benthamiana when the plants were pretreated with crude leaf extract of Capsicum chinense ‘Sy-2’.. C. chinense leaves were revealed to contain strong RNA-digesting activity.. Abstract Several studies have identified active antiviral and antiviroid agents in plants.

    Article Title: Development of an assay for the detection and quantification of the measles virus nucleoprotein (N) gene using real-time reverse transcriptase PCR.
    Article Snippet: .. The reverse transcription mixture contained 10 ml template RNA from the throat swab specimens, 4 ml random hexamer (20 pmol), 4 ml 56 RT-PCR buffer, 1 ml PrimeScript RT Enzyme Mix I (Takara) containing 10 units RNase inhibitor ml21 (Applied Biosystems) and 1 ml DNase- and RNase-free distilled water. ..

    Article Title: A Novel Enzootic Nasal Tumor Virus Circulating in Goats from Southern China
    Article Snippet: .. One step RT-PCRs were performed with a final reaction volume of 50 μL, containing 25 μL 2 × RT-PCR buffer (Takara, Inc., Dalian, Liaoning, China), 1 μL Enzyme Mix (including reverse transcriptase and DNA polymerase), 5 μL viral RNA, 1 μL each of the primers (10 pmol), and 17 μL RNase-free water. ..

    Article Title: Primary surveys on molecular epidemiology of bovine viral diarrhea virus 1 infecting goats in Jiangsu province, China
    Article Snippet: Total RNA was extracted from the serum samples using TRIzol Reagent (Invitrogen), and suspended in 20 μL of ultrapure water, following the manufacturer’s recommendations. .. RT-PCR was carried out in a 50 μL reaction mixture containing 1× RT-PCR buffer (TAKARA, Bio, Inc.), 20 pM of Panpesti generic primers targeting 5′-UTR, 2 U of one-step Enzyme Mix (TAKARA, Bio, Inc.) and 4 μL of RNA for the expected product sizes of 290 bp [ ], the reaction was run in a thermocycler (Mjmini, BIO-RAD) according to the following program: reverse transcription at 50 °C for 30 min, denaturation at 95 °C for 5 min, 35 cycles at 94 °C for 30 s, 54 °C for 30 s and 72 °C for 45 s, and termination with a final extension of 10 min at 72 °C. ..

    Article Title: Analysis on the complete genome of a novel caprine parainfluenza virus 3.
    Article Snippet: Article history: Received 30 September 2015 Received in revised form 25 November 2015 Accepted 25 November 2015 Available online 26 November 2015 Parainfluenza virus type 3 (PIV3) is one of the most important viral respiratory pathogens for humans and for many animals.. One unique caprine PIV3 (CPIV3) strain named JS2013 was isolated in Chinese goat flocks with respiratory diseases in 2013.. Now, the complete genome sequence of the strain JS2013 had been determined.

    Article Title: Parallel measurement of transcriptomes and proteomes from same single cells using nanodroplet splitting
    Article Snippet: .. Following the transfer of samples into a 384-well plate containing RT-PCR buffer with 3’ SMART-Seq CDS Primer IIA (SMART-Seq® v4 PLUS Kit, TaKaRa, cat# R400753); the samples were immediately denatured at 72oC for 3 min and chilled on ice for at least 2 min. Full length cDNA was generated by adding RT mix to each tube and incubating at 42oC for 90 min; followed by heat inactivation at 70oC for 10 min. 18 cycles of cDNA amplification were done to generate enough cDNA for template library according to SMART-Seq® v4 PLUS Kit instruction. .. The SMART-Seq Library Prep Kit and Unique Dual Index Kit (TaKaRa, cat# R400745) were used to generate barcoded template library for sequencing.

    Sterility:

    Article Title: Digestion of chrysanthemum stunt viroid by leaf extracts of Capsicum chinense indicates strong RNA-digesting activity.
    Article Snippet: Key message CSVd could not infect Nicotiana benthamiana when the plants were pretreated with crude leaf extract of Capsicum chinense ‘Sy-2’.. C. chinense leaves were revealed to contain strong RNA-digesting activity.. Abstract Several studies have identified active antiviral and antiviroid agents in plants.

    Quantitative RT-PCR:

    Article Title: Digestion of chrysanthemum stunt viroid by leaf extracts of Capsicum chinense indicates strong RNA-digesting activity.
    Article Snippet: Key message CSVd could not infect Nicotiana benthamiana when the plants were pretreated with crude leaf extract of Capsicum chinense ‘Sy-2’.. C. chinense leaves were revealed to contain strong RNA-digesting activity.. Abstract Several studies have identified active antiviral and antiviroid agents in plants.

    Reverse Transcription:

    Article Title: Development of an assay for the detection and quantification of the measles virus nucleoprotein (N) gene using real-time reverse transcriptase PCR.
    Article Snippet: .. The reverse transcription mixture contained 10 ml template RNA from the throat swab specimens, 4 ml random hexamer (20 pmol), 4 ml 56 RT-PCR buffer, 1 ml PrimeScript RT Enzyme Mix I (Takara) containing 10 units RNase inhibitor ml21 (Applied Biosystems) and 1 ml DNase- and RNase-free distilled water. ..

    Article Title: A Novel Enzootic Nasal Tumor Virus Circulating in Goats from Southern China
    Article Snippet: .. One step RT-PCRs were performed with a final reaction volume of 50 μL, containing 25 μL 2 × RT-PCR buffer (Takara, Inc., Dalian, Liaoning, China), 1 μL Enzyme Mix (including reverse transcriptase and DNA polymerase), 5 μL viral RNA, 1 μL each of the primers (10 pmol), and 17 μL RNase-free water. ..

    Article Title: Primary surveys on molecular epidemiology of bovine viral diarrhea virus 1 infecting goats in Jiangsu province, China
    Article Snippet: Total RNA was extracted from the serum samples using TRIzol Reagent (Invitrogen), and suspended in 20 μL of ultrapure water, following the manufacturer’s recommendations. .. RT-PCR was carried out in a 50 μL reaction mixture containing 1× RT-PCR buffer (TAKARA, Bio, Inc.), 20 pM of Panpesti generic primers targeting 5′-UTR, 2 U of one-step Enzyme Mix (TAKARA, Bio, Inc.) and 4 μL of RNA for the expected product sizes of 290 bp [ ], the reaction was run in a thermocycler (Mjmini, BIO-RAD) according to the following program: reverse transcription at 50 °C for 30 min, denaturation at 95 °C for 5 min, 35 cycles at 94 °C for 30 s, 54 °C for 30 s and 72 °C for 45 s, and termination with a final extension of 10 min at 72 °C. ..

    Random Hexamer:

    Article Title: Development of an assay for the detection and quantification of the measles virus nucleoprotein (N) gene using real-time reverse transcriptase PCR.
    Article Snippet: .. The reverse transcription mixture contained 10 ml template RNA from the throat swab specimens, 4 ml random hexamer (20 pmol), 4 ml 56 RT-PCR buffer, 1 ml PrimeScript RT Enzyme Mix I (Takara) containing 10 units RNase inhibitor ml21 (Applied Biosystems) and 1 ml DNase- and RNase-free distilled water. ..

    Generated:

    Article Title: Parallel measurement of transcriptomes and proteomes from same single cells using nanodroplet splitting
    Article Snippet: .. Following the transfer of samples into a 384-well plate containing RT-PCR buffer with 3’ SMART-Seq CDS Primer IIA (SMART-Seq® v4 PLUS Kit, TaKaRa, cat# R400753); the samples were immediately denatured at 72oC for 3 min and chilled on ice for at least 2 min. Full length cDNA was generated by adding RT mix to each tube and incubating at 42oC for 90 min; followed by heat inactivation at 70oC for 10 min. 18 cycles of cDNA amplification were done to generate enough cDNA for template library according to SMART-Seq® v4 PLUS Kit instruction. .. The SMART-Seq Library Prep Kit and Unique Dual Index Kit (TaKaRa, cat# R400745) were used to generate barcoded template library for sequencing.

    Amplification:

    Article Title: Parallel measurement of transcriptomes and proteomes from same single cells using nanodroplet splitting
    Article Snippet: .. Following the transfer of samples into a 384-well plate containing RT-PCR buffer with 3’ SMART-Seq CDS Primer IIA (SMART-Seq® v4 PLUS Kit, TaKaRa, cat# R400753); the samples were immediately denatured at 72oC for 3 min and chilled on ice for at least 2 min. Full length cDNA was generated by adding RT mix to each tube and incubating at 42oC for 90 min; followed by heat inactivation at 70oC for 10 min. 18 cycles of cDNA amplification were done to generate enough cDNA for template library according to SMART-Seq® v4 PLUS Kit instruction. .. The SMART-Seq Library Prep Kit and Unique Dual Index Kit (TaKaRa, cat# R400745) were used to generate barcoded template library for sequencing.



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